Identification of differential expressed genes after SP1 or SP3 knockdown in colorectal cancer cells

SP1 and SP3 have been reported to play an critical role in the pregression of tumors. In order to elucidate the molecular mechanism of SP1 and SP3 in colorectal cancer, RNA-seq analysis was performed on SP1 and SP3 knockdown and control HCT116 cells. HCT116 cells with SP1 or SP3 knockdown were generated by siRNAs.

已有研究表明，SP1与SP3在肿瘤进展过程中发挥关键作用。为阐明SP1与SP3在结直肠癌中的分子机制，本研究对SP1、SP3基因敲低组及对照组HCT116细胞进行了RNA测序（RNA-seq）分析。本研究通过小干扰RNA（siRNAs）构建了SP1或SP3基因敲低的HCT116细胞模型。