Genomic Characterization of Bladder Cancer Cell Lines using CGH Arrays

Genomic rearrangements involving different chromosome translocation in bladder cancer cell lines and in primary tumor samples DNA extracted from 3 Bladder Cancer Cell lines (SW1710, JON, RT-4), and 1 bladder cancer (372C) in T3 Tumor stage and Grade 3 primary sample, were processed, Cy5 labeled and hybridized on Agilent CGH Arrays. As control DNA we used Cy3 labeled probes prepared starting from a pool of female DNA obtained mixing the DNA extracted from 20 healthy donors.

本研究针对膀胱癌细胞系与原发性肿瘤样本中涉及不同染色体易位的基因组重排构建数据集：提取3株膀胱癌细胞系（SW1710、JON、RT-4）以及1份T3分期、3级的原发性膀胱肿瘤样本（372C）的基因组DNA，对提取的DNA进行Cy5标记后，在安捷伦（Agilent）CGH芯片上完成杂交实验。本研究的对照DNA采用Cy3标记的探针，该探针通过混合20名健康供者提取的基因组DNA制备的女性DNA池制备得到。