Calcein release from LMDP-lipo into cells.

DiI-labeled and calcein encapsulated Control-lipo (EPC/DOPE/CHEMS) (a), LMDP-lipo (EPC/DOPE/CHEMS/5 mol% LMDP) (b), LMDP-lipo in the presence of 50 µM DAPT (c), LMDP-lipo in the presence of 0.4 M sucrose (d), LMDP-lipo in the presence of 2.5 mM amiloride (e) and LMDP-lipo in the presence of 5 µg/mL FilipinIII (f) were added to A549 cells and incubated at 37°C for 1 h. The intracellular location of CM-DiI (red) and calcein (green) was observed by CLSM. Red signal indicates liposomes. Scale bars, 10 µm.

将DiI标记且包裹钙黄绿素的对照脂质体（EPC/DOPE/CHEMS）（a组）、LMDP脂质体（EPC/DOPE/CHEMS/5 mol% LMDP）（b组）、添加50 μM DAPT的LMDP脂质体（c组）、添加0.4 M蔗糖的LMDP脂质体（d组）、添加2.5 mM阿米洛利的LMDP脂质体（e组）以及添加5 μg/mL Filipin III（菲洛品III）的LMDP脂质体（f组）分别加入A549细胞中，于37℃孵育1小时。通过共聚焦激光扫描显微镜（Confocal Laser Scanning Microscopy, CLSM）观察CM-DiI（红色信号）与钙黄绿素（绿色信号）的细胞内定位，其中红色信号代表脂质体。比例尺为10 μm。