Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR libraries

This project developed a method to engineer dozens to hundreds of variants in parallel and produce cell lines differing genetically only by CRISPR-induced mutations.本项目开发了一种可并行构建数十至数百个基因变体的方法，可获得仅存在成簇规律间隔短回文重复序列（CRISPR）诱导突变遗传差异的细胞系。