Total RNA sequencing of the liver tissues of Oreochromis niloticus (Nile Tilapia) and of Pundamilia nyerei

Cichlids fishes exhibit extensive phenotypic diversification and speciation. In this study we integrate transcriptomic and proteomic signatures from two cichlids species, identify novel open reading frames (nORFs) and perform evolutionary analysis on these nORF regions. We embark comparative transrcriptomics and proteogenomic analysis of two metabolically active tissues, the testes and liver, of two cichlid species Oreochromis niloticus (Nile tilapia, ON) and Pundamilia nyererei (Makobe Island, PN). Our results suggest that the time scale of speciation of the two species can be better explained by the evolutionary divergence of these nORF genomic regions. Total RNA was extracted from liver tissue of two cichlid fishes: Oreochromis niloticus and Pundamilia nyererei; three samples each.

慈鲷鱼类展现出广泛的表型分化与物种形成现象。本研究整合两种慈鲷的转录组与蛋白质组特征，鉴定新型开放阅读框（novel open reading frames，nORFs），并针对这些nORF基因组区域开展进化分析。本研究针对两种慈鲷——尼罗罗非鱼（Oreochromis niloticus，Nile tilapia，ON）与采集自马科贝岛的尼氏朴丽鱼（Pundamilia nyererei，PN）——的两种代谢活跃组织（睾丸与肝脏）开展比较转录组学与蛋白质基因组学分析。研究结果显示，这两个物种的物种形成时间尺度，可通过这些nORF基因组区域的进化分化得到更合理的解释。本研究从两种慈鲷的肝脏组织中提取总RNA，每个物种各设置3个生物学重复样本。