Transcriptome data from Eomes-overexpressing Th17 cells. Transcriptome data from Eomes-overexpressing Th17 cells

Th17 cells were sorted ex vivo from PB of healthy donors as CD4+CD161+CCR6+CXCR3-. Following, cells were transduced with a lentiviral vector carrying the Eomes gene or with an empty vector. Infected cells were then enriched by MACS separation using the reporter gene NGFR as selection marker. Finally, cells were frozen for RNA analysis. Overall design: Th17 cells from one donor were transduced with a lentiviral vector carrying the Eomes gene or with a control vector. Two technical replicates were performed.

从健康供者的外周血（peripheral blood, PB）中，以CD4+CD161+CCR6+CXCR3-为表面标记离体分选出Th17细胞。随后，将该批细胞分别用携带Eomes基因的慢病毒载体或空载对照载体进行转导。之后，以报告基因神经生长因子受体（Nerve Growth Factor Receptor, NGFR）作为筛选标记，通过磁激活细胞分选（Magnetic Activated Cell Sorting, MACS）对转导后的细胞进行富集。最终，将细胞冻存以备开展RNA分析。
实验总体设计：从单名健康供者获取的Th17细胞被分为两组，分别使用携带Eomes基因的慢病毒载体与空载对照载体进行转导，每组均设置2个技术重复。