GRHL and PGR control WNT4 expression in the mammary gland via 3D 2 looping of conserved and species-specific enhancers [BC44 HC11 C57MG RNA-seq]

This dataset belongs to a study in which we functionally dissect the cis-acting enhancer network and spatiotemporal transcriptional mechanisms that regulate WNT4 expression in the mouse mammary gland and the human breast. As part of these efforts, we performed transcriptional profiling of BC44 and HC11, which differentially express Wnt4. This bulk RNAseq dataset also includes a third mouse mammary epithelial cell line, C57MG and should serve as a reference atlas for differential gene expression between these three cell lines. Overall design: Bulk RNA sequencing of untreated BC44, HC11 and C57MG mouse mammary epithelial cell lines.

本数据集源自一项研究，该研究旨在对调控小鼠乳腺与人类乳腺中WNT4表达的顺式作用增强子（cis-acting enhancer）网络及时空转录调控机制进行功能解析。作为该研究的组成部分，我们对差异表达WNT4的BC44与HC11细胞系开展了转录谱分析。本批量RNA测序（bulk RNA-seq）数据集还纳入了第三种小鼠乳腺上皮细胞系C57MG，可作为这三种细胞系间差异基因表达分析的参考图谱。整体实验设计：对未处理的BC44、HC11及C57MG三种小鼠乳腺上皮细胞系进行批量RNA测序。