Growth of Burkholderia sacchari LFM 101 cultivated in glucose, sucrose and glycerol at different temperatures

ABSTRACT Polyhydroxyalkanoates (PHAs) have attracted major industrial interest as alternatives to conventional plastics. They are produced by several bacteria as cytoplasmic inclusions when nutrients are in limited supply. Among the many factors influencing bacterial growth, the effect of temperature on both specific growth rates and growth yields in terms of carbon source intake is of considerable interest. This study aimed to evaluate the influence of the bacterium Burkholderia sacchari LFM 101 on growth and PHA production, using glucose, sucrose or glycerol as a carbon source, at 30 and 35 °C. The results showed that B. sacchari cultured with glucose at 35 °C presented both higher productivity and polymer yield in dried cell mass. There were no differences in growth rates (μmax) in sucrose and glucose. The growth conditions studied were not favorable to glycerol consumption due to limitations in the energy supply from glycerol.

摘要 聚羟基脂肪酸酯（Polyhydroxyalkanoates, PHAs）作为传统塑料的替代材料，已获得工业界的广泛关注。该类聚合物可由多种细菌在营养受限条件下合成，并以细胞质内含物的形式存在。在影响细菌生长的诸多因素中，温度对细菌比生长速率及基于碳源摄取的生长得率的影响备受关注。本研究以葡萄糖、蔗糖或甘油为碳源，分别在30 ℃与35 ℃条件下培养伯克霍尔德氏菌（Burkholderia sacchari）LFM 101，旨在评估该菌株的生长情况与PHA合成能力。结果显示，以葡萄糖为碳源、35 ℃下培养的B. sacchari，其干细胞重的生产效率与聚合物得率均更高。以蔗糖和葡萄糖为碳源时，细菌的最大比生长速率（μmax）无显著差异。本次研究采用的培养条件不利于甘油的代谢利用，这归因于甘油代谢过程中的能量供应受限。