Whole genome sequence and annotation of Penstemon davidsonii

Penstemon is the most speciose flowering plant genus endemic to North America. Penstemon species' diverse morphology and adaptation to various environments have made them a valuable model system for studying evolution, but the absence of publicly available reference genomes limits possible research directions. Here we report the first reference genome assembly and annotation for Penstemon davidsonii. Using PacBio long-read sequencing and Hi-C scaffolding technology, we constructed a de novo reference genome of 437,568,744 bases, with a contig N50 of 40 Mb and L50 of 5. The annotation includes 18,199 gene models, and both the genome and transcriptome assembly contain over 95% complete eudicot BUSCOs. This genome assembly will serve as a valuable reference for studying the evolutionary history and genetic diversity of the Penstemon genus.

钓钟柳属（Penstemon）是北美特有物种多样性最高的开花植物属。钓钟柳属物种丰富的形态多样性与对各类生境的适应性，使其成为研究演化过程的极具价值的模式系统，但目前公开可用的参考基因组的缺失限制了相关研究方向的开展。本研究首次报道了戴维森钓钟柳（Penstemon davidsonii）的参考基因组组装与注释结果。本研究利用PacBio长读长测序与Hi-C支架组装技术，构建了总长度为437,568,744碱基的从头组装参考基因组，其重叠群N50（contig N50）为40 Mb，L50为5。注释结果共包含18,199个基因模型，且该基因组与转录组组装结果的真双子叶植物BUSCO（Benchmarking Universal Single-Copy Orthologs）完整度均超过95%。该基因组组装结果将为钓钟柳属的演化历史与遗传多样性研究提供极具价值的参考资源。