Dual-species microarray for assessing gene expression in stromal microenvironment, cancer cells and their interactions (v2 array). Dual-species microarray for assessing gene expression in stromal microenvironment, cancer cells and their interactions (v2 array)

Design a human-mouse dual-species microarray to provide a technology for investigation of cancer-stromal interaction in xenograft model. The 2 reference RNA were used to evaluate and identify probes with potential the cross-species hybridization, so that we can label these probes and exclude them before analysis of biological samples/data. Overall design: Reference RNA from human and mouse were labeled using Cy3. Samples were hybridized on customized-commercial array with Agilent probes and user-designed species-specific probes (Human-mouse dual-species array HomoMus_v2). This array is designed and improved from hte first version GPL10714

本研究构建人-鼠双物种微阵列，为异种移植模型中癌症-基质相互作用的研究提供技术支撑。选取两份参考RNA，用于评估并鉴定存在跨物种杂交潜力的探针，以便在后续生物样本/数据分析前对这类探针进行标记并剔除。实验整体设计：采用Cy3对人源与鼠源参考RNA进行标记，将样本与定制化商用微阵列进行杂交；该阵列包含安捷伦（Agilent）探针以及用户自定义的物种特异性探针（人-鼠双物种微阵列HomoMus_v2）。此微阵列基于初代版本GPL10714优化设计而来。