Exon array analysis of N2A cells after knocking down FUS

To analyze the role of Fus, Ewsr1, and Taf15 in alternative RNA processing, we performed exon array analysis in N2A cells using exon arrays. N2A cells were transfected with siRNA using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions. We analyzed total RNA of N2A cells transfected with siRNA against mouse Fus, combination of Fus, Ewsr1 and Taf15, or control. RNA was harvested 48 hours after transfection.

为探究Fus、Ewsr1与Taf15在可变RNA加工过程中的作用，我们在N2A细胞中利用外显子芯片（exon array）开展外显子阵列分析。我们采用Lipofectamine RNAiMAX（Life Technologies公司产品），严格按照制造商说明书将小干扰RNA（siRNA）转染至N2A细胞内。我们分别对转染靶向小鼠Fus的siRNA、同时靶向Fus、Ewsr1与Taf15的混合siRNA，以及阴性对照siRNA的N2A细胞总RNA进行分析。转染48小时后收集细胞总RNA。