Single-cell repertoire and transcriptome sequencing reveals clonally expanded and transcriptionally distinct lymphocytes in aged CNS

Single-cell repertoire and transcriptome sequencing reveals clonally expanded and transcriptionally distinct lymphocytes in aged CNS. Gene expression and immune receptor repertoire sequencing was performing for both B and T cells. This dataset contains the VDJ sequencing information for the four samples. Each B cell and T cell library was sequenced across four lanes. Files with _WT_ in their name correspond to the young (4-6 week B6 mice) Files with _12_ in their name before the BDJ or VDJ text correspond to the 12-month-old cohort. Files with _18_ in their name before the BDJ or VDJ text correspond to the 18-month-old cohort in which four brains were pooled. Files with 4_18_ in their name before the BDJ or VDJ text correspond to the 18-month-old mouse that was processed and sequenced alone. The L001 - L004 in the file names indicates the sequencing lane. Samples with BDJ correspond to the B cell repertoire library (B cell VDJ). Samples with TDJ correspond to the T cell repertoire library (T cell VDJ).单细胞免疫组库与转录组测序揭示衰老中枢神经系统（Central Nervous System, CNS）中存在克隆扩增且转录特征异质性的淋巴细胞。本研究对B细胞与T细胞开展了基因表达及免疫受体组库测序。本数据集包含4个样本的VDJ测序数据。每个B细胞及T细胞文库均通过4个测序泳道完成测序。文件名含_WT_的样本对应年轻小鼠（4-6周龄B6小鼠）；在BDJ或VDJ字段前带有_12_的文件，对应12月龄小鼠队列；在BDJ或VDJ字段前带有_18_的文件，对应将4个脑组织混合制备的18月龄小鼠队列；在BDJ或VDJ字段前带有4_18_的文件，对应单独处理并测序的单只18月龄小鼠。文件名中的L001至L004代表测序泳道。带有BDJ标识的样本为B细胞组库文库（B细胞VDJ测序文库）；带有TDJ标识的样本为T细胞组库文库（T细胞VDJ测序文库）。