Stem cell lines of the early mouse embryo

Expression profiling of stem cell lines derived from the early embryo representing the trophoblast, primitive endoderm, early epiblast (inner cell mass E3.5) and late post-implantation epiblast (E5.5). Cells were grown without feeders and harvested. Comparisons were made to provide evidence of unique gene expression between the cell lines. Specifically, pre-implantation (ICM, epiblast and primitive endoderm, and trophoblast), as well as pre-implantation and post-implantation epiblasts.

本数据集针对源自早期胚胎的干细胞系开展基因表达谱分析（expression profiling），这些细胞系分别代表滋养层细胞（trophoblast）、原始内胚层（primitive endoderm）、早期上胚层（epiblast，即E3.5时期的内细胞团（inner cell mass））以及植入后晚期上胚层（E5.5时期）。实验所用细胞均在无饲养层培养体系中增殖并收获。通过组间比较，以揭示不同干细胞系间独特的基因表达特征。具体而言，本次对比涵盖植入前阶段的细胞（内细胞团、上胚层、原始内胚层及滋养层细胞），以及植入前与植入后两个时期的上胚层。