Transcriptional profiling of single cells from epithelial organoids modelling Intestinal tumorigenesis

To chart cell state dynamics across the malignancy continuum of colorectal cancer, wild type organoids (WT), ApcKO (A) and ApcKO::KrasG12D::Smad4KO::Trp53KO (AKSP) tumoroids were subjected to single cell RNA-seq. Isogenic lines with the indicated genotypes were collected for s.cRNA-seq before reaching confluency to minimize cell death.

为解析结直肠癌恶性进展连续体中的细胞状态动态变化，我们对野生型类器官（wild type organoid，WT）、Apc基因敲除类器官（ApcKO，记为A）以及ApcKO::KrasG12D::Smad4KO::Trp53KO（AKSP）肿瘤类器官开展了单细胞RNA测序（single-cell RNA-seq）。为尽可能减少细胞死亡，我们在细胞达到汇合度前收集了携带指定基因型的同基因系样本用于单细胞RNA测序。