Expression data from rat with different quantities of PS. Rattus norvegicus

Paradoxical sleep function remains unknown although several studies indicate that it might play a role in learning and memory. To investigate what modifications paradoxical sleep may bring at the molecular level in neocortex and in hippocampal formation, we profiled gene expression in these structures in rats with different quantities of PS by cDNA microarrays approach. Overall design: The first group of rats (n=12) was deprived of PS (PSD) by the multiple platform method during 78h, the second group of rats was allowed to recover from this deprivation (PSR) during 6 hours (n=12), the third group of rats remained in their home cage during all the protocol PSC (n=12). All animals were euthanasied at 16h. An equal mass amount of total RNA from the right neocortex and hippocampal formation was pooled from two sets of 6 rats from each experimental group (PSC, PSD, PSR). For the first set of animals, two independent pools of total RNA from both structures were made for each condition to control for the technical variability of the microarray method.

尽管多项研究表明异相睡眠（paradoxical sleep）可能参与学习与记忆过程，但其生理功能至今仍未明确。为探究异相睡眠可能在新皮层（neocortex）与海马结构（hippocampal formation）的分子层面引发的调控变化，本研究采用cDNA微阵列（cDNA microarray）技术，对不同异相睡眠时长的大鼠的上述脑结构开展基因表达谱分析。
整体实验设计如下：第一组大鼠（n=12）采用多平台法在78小时内剥夺异相睡眠，记为PSD组；第二组大鼠在剥夺结束后恢复6小时异相睡眠，记为PSR组（n=12）；第三组大鼠全程置于饲养笼中，作为空白对照组，记为PSC组（n=12）。所有动物均在实验周期结束后16小时实施安乐死。
从每个实验组（PSC、PSD、PSR）的两组各6只大鼠中，分别提取右侧新皮层与海马结构的总RNA，按等量质量混合制成混合样本；即每个实验组制备两份独立的混合样本，每份样本对应6只大鼠的混合RNA。对于第一批次的动物，每个实验条件下均制备两份上述脑结构的总RNA混合样本，以控制微阵列实验的技术变异。