Smad4 restricts injury-provoked biliary proliferation and carcinogenesis (Smad4_floxed_vs_WT_RNAseq)

To understand the processes regulated by Smad4 in reactive cholangiocytes, Ctl and Smad4del mice were provided DDC diet for 2 weeks after which reactive cholangiocytes were isolated from digested injured mouse liver via FACS followed by RNA amplification and sequencing analysis. Gene set enrichment analysis of Smad4-perturbed reactive cholangiocytes implicates Smad4 in proliferative, metabolic, and inflammatory pathways. Comparative gene expression profiling analysis of RNA-seq data for reactive cholangiocytes with and without Smad4

为探究Smad4在反应性胆管上皮细胞（reactive cholangiocytes）中调控的生物学过程，本研究为对照组（Ctl）与Smad4敲除（Smad4del）小鼠提供DDC饮食造模2周；随后通过流式细胞术（FACS）从消化后的受损小鼠肝脏中分离反应性胆管上皮细胞，继而开展RNA扩增与测序分析。对经Smad4扰动的反应性胆管上皮细胞进行基因集富集分析（Gene Set Enrichment Analysis）结果表明，Smad4参与增殖、代谢及炎症通路的调控。本研究针对携带与缺失Smad4的反应性胆管上皮细胞的RNA测序（RNA-seq）数据开展比较基因表达谱分析。