COLO 320 (Human colorectal cancer cell line) xenograft: Control (Xenograft without rat mesenchymal stem cells (MSCs)) vs Xenograft with rat MSCs

Transcriptional profiling of COLO 320 xenograft tumor cells comparing control COLO 320 xenograft without co-implanted rat MSCs with COLO 320 xenograft with co-implanted rat MSCs. The latter makes co-implanted MSCs visualization possible by using MSCs labeled by GFP under FACS and single cell microscopy. Two-condition experiment, COLO 320 xenograft without rat MSCs [COLO320 MSC(-)] vs. COLO 320 xenograft with rat MSCs [COLO320 MSC(+)]. Biological replicates: 1 control, 1 sample, paired xengraft tumor cells grown and harvested from the same mouse host. One replicate per array.

本数据集为COLO 320异种移植瘤细胞的转录组谱分析实验，对比两组样本：未共植入大鼠间充质干细胞（Mesenchymal Stem Cells, MSCs）的COLO 320异种移植瘤，与共植入大鼠MSCs的COLO 320异种移植瘤。后者可通过使用绿色荧光蛋白（Green Fluorescent Protein, GFP）标记的MSCs，借助荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）与单细胞显微镜实现共植入MSCs的可视化。本实验采用双组对照设计：无大鼠MSCs共植入的COLO 320异种移植瘤[COLO320 MSC(-)] 与有大鼠MSCs共植入的COLO 320异种移植瘤[COLO320 MSC(+)]。生物学重复设置为：1个对照样本与1个处理样本，两组均为从同一小鼠宿主中培养并收获的配对异种移植瘤细胞，每个基因芯片阵列对应一个生物学重复。