High-throughput RNA-sequencing transcriptome analysis of ABC-DLBCL revealed new targets genes. High-throughput RNA-sequencing transcriptome analysis of ABC-DLBCL revealed new targets genes

Purpose: we searched for new potential druggable therapeutic targets through the description of high or low abundance transcripts that might be critical for ABC DLBCL cells. Overall design: The bulk of DLBCL were categorized in Cell-Of-Origen (COO) by Nanostring platform prior to deep transcriptome analysis by RNA sequencing (RNA-seq). After comparing GCB and ABC transcriptomes, a set of 79 genes were found differentially expressed (DE). By performing Gene Set Enrichment Analysis (GSEA), two main pathways were enriched and were related to metabolism and immune pathways.

研究目的：本研究通过对可能对活化B细胞样弥漫大B细胞淋巴瘤（ABC DLBCL）细胞至关重要的高丰度与低丰度转录本进行表征分析，旨在发掘全新的潜在可药用治疗靶点。
整体实验设计：在通过RNA测序（RNA-seq）开展深度转录组分析前，先采用Nanostring平台对多数弥漫大B细胞淋巴瘤样本进行起源细胞（Cell-Of-Origen, COO）分型。通过比较生发中心B细胞样（GCB）与ABC DLBCL的转录组特征，筛选得到79个差异表达（differentially expressed, DE）基因。后续通过基因集富集分析（Gene Set Enrichment Analysis, GSEA）发现两条主要富集通路，分别与代谢及免疫通路相关。