Regulation of Xylem transcriptome by water supply and potassium nutrition

(1) Wood production in fast-growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effect of these two limiting factors are still unknown. E. grandis trees were submitted to four combinations of K fertilization and water supply. Transcriptomic analyses (RNAseq) were performed to study the crosstalk responses triggered by water availability and potassium supply at the level of gene expression. Three sequencing libraries per treatment were generated and sequenced (Illumina, 150 bp paired-end reads) by the Genotoul GeT platform (https://get.genotoul.fr/en/; France) using the HiSeq3000 (Illumina). An average of 46 million reads per library was generated, >90% were mapped to E. grandis reference genome (v1.1, https://phytozome.jgi.doe.gov/) using TopHat2 (Kim et al., 2013).

(1) 速生巨桉（Eucalyptus grandis）的木材产量高度依赖钾（K）肥施用与水分供应，但针对这两种限制因子共同调控木材形成的分子机制，目前仍未明确。研究人员对巨桉植株设置了4种钾肥施用与水分供应的组合处理。为探究水分有效性与钾素供给共同触发的基因表达层面的互作响应，本研究开展了转录组测序（RNAseq）分析。每个处理组构建3个生物学重复测序文库，由法国Genotoul GeT平台（https://get.genotoul.fr/en/）采用Illumina HiSeq3000进行测序，测序模式为150 bp双端读长。每个文库平均产出4600万条测序reads，其中超过90%的序列可通过TopHat2（Kim等，2013）比对至巨桉参考基因组（v1.1，https://phytozome.jgi.doe.gov/）。