Comparative transcriptomics (mRNAseq) of fruit fly heads exhibiting chronic proteostasis dysregulation in neuronal circuits.

This mRNA sequencing project aims to elucidate the transcriptomic repercussions of dysregulated neuronal proteostasis and the accumulation of transcription factors, including Xrp1 and crc, in condensates documented in multiple fruit fly mutant lines. Experimental design: Comparative gene expression from control (w1118) and two mutant (aln2 and norpA) adult Drosophila melanogaster heads. Mutant lines used were homozygous for the aln2 and norpA alleles and showed proteostasis dysregulation in neurons. All flies were reared unstressed on standard food, at 25C, and a 12:12 light-dark cycle then aged 3-5 days post eclosion. Three biological replicate single-end, 100bp libraries were prepared for each genotype using a TruSeq Stranded mRNA Library Prep Kit and sequenced on an Illumina NextSeq 2000 utilizing a P2 flowcelll.

本mRNA测序（mRNA sequencing）项目旨在阐明神经元蛋白质稳态（proteostasis）失调，以及Xrp1与crc等转录因子在多种果蝇突变株系的凝聚体（condensates）中异常积累所引发的转录组层面的效应。实验设计：对对照组（w1118）与两种突变株系（aln2、norpA）的成年黑腹果蝇（Drosophila melanogaster）头部进行比较基因表达分析。所用突变株系均为aln2与norpA等位基因的纯合子，且表现出神经元蛋白质稳态失调。所有果蝇均在无胁迫条件下饲养于标准培养基，培养温度为25℃，光照周期为12小时光照:12小时黑暗，羽化后饲养3至5天。每种基因型均设置3个生物学重复，采用TruSeq链特异性mRNA文库制备试剂盒构建单端100bp测序文库，并在搭载P2流动池的Illumina NextSeq 2000测序平台上完成测序。