Drosophila serrata mutation accumulation lines: Phenotypic data on survival following infection with Drosophila C virus and reproduction

The impact of selection on host immune function genes has been widely documented. However, it remains essentially unknown how mutation influences the quantitative immune traits that selection acts on. Applying a classical mutation accumulation (MA) experimental design in Drosophila serrata, we found the mutational variation in susceptibility (median time of death, LT50) to Drosophila C virus (DCV) was of similar magnitude to that reported for intrinsic survival traits. Mean LT50 did not change as mutations accumulated, suggesting no directional bias in mutational effects. Maintenance of genetic variance in immune function is hypothesised to be influenced by pleiotropic effects on immunity and other traits that contribute to fitness. To investigate this, we assayed female reproductive output for a subset of MA lines with relatively long or short survival times under DCV infection. Longer survival time tended to be associated with lower reproductive output, suggesting that mutations affec..., This project first determined that D. serrata is a susceptible host of the Drosophila C virus. Second, survival time following DCV infection was measured for a panel of 65 inbred lines that differed from one another by fixation of spontaneous mutations over 30 generations , and that data used to determine how mutations affected DCV susceptibility. Finally, for a subset of lines with extreme differences in survival time, we also measured female offspring production to determine whether mutations affecting immune function also affected productivity., , # Drosophila serrata Mutation Accumulation lines: Phenotypic data on survival following infection with Drosophila C virus and reproduction

[https://doi.org/10.5061/dryad.k6djh9wg3](https://doi.org/10.5061/dryad.k6djh9wg3)

## Description of the data and file structure

List of files and their use.

1\. `DCV vs PBS and Sex.csv`: file contains data from first (prelim) experiment determining susceptibility of D. serrata to DCV infection. Data is day of death for each fly in each vial; further details in the `DCV vs PBS and Sex README.txt`. This data is analysed using `DCV PBS Sex Survival Curve Analyses.R` file to generate both main text and supplementary results.

2\. `Expt 2 MA LT50 input data.csv`: the data from experiment in which 4 (12) replicates vials of 7 females from each of 65 MA lines (their Ancestor) were injected with DCV, and survival recored. Data formatted as the number of flies alive and number dead on each day post injection. Full data description in 'Expt 2 MA LT50 inp...

宿主免疫功能基因所受选择的影响已被广泛报道。然而，突变如何作用于选择所靶向的数量免疫性状，目前仍基本未知。本研究在锯缘果蝇（Drosophila serrata）中采用经典的突变积累（Mutation Accumulation, MA）实验设计，结果发现，果蝇C病毒（Drosophila C virus, DCV）易感性（死亡中值时间LT50）的突变变异幅度，与已报道的固有存活性状的变异幅度相近。随着突变的积累，平均LT50未发生显著变化，提示突变效应不存在方向性偏倚。学界假说认为，免疫功能遗传变异的维持，可能受到免疫相关性状及其他贡献于适合度的性状的多效性效应影响。为验证这一假说，我们对DCV感染后存活时间相对较长或较短的一部分MA品系的雌性繁殖产出进行了检测。结果显示，存活时间更长的个体往往伴随更低的繁殖产出，提示突变可影响...

本研究首先明确了锯缘果蝇（Drosophila serrata）是果蝇C病毒的易感宿主。其次，针对65个经30代自发突变固定而彼此存在差异的近交系MA品系，我们检测了DCV感染后的存活时间，并利用该数据明确了突变对DCV易感性的影响。最后，针对存活时间存在极端差异的一部分品系，我们还检测了雌性后代产量，以探究影响免疫功能的突变是否同时影响繁殖能力。

# 锯缘果蝇突变积累品系：果蝇C病毒感染后存活与繁殖的表型数据
https://doi.org/10.5061/dryad.k6djh9wg3

## 数据与文件结构说明
文件列表及用途如下：
1. `DCV vs PBS and Sex.csv`：包含首次预实验的数据，用于明确锯缘果蝇对DCV感染的易感性。数据内容为每培养管中每只果蝇的死亡天数；详细说明见`DCV vs PBS and Sex README.txt`。该数据通过`DCV PBS Sex Survival Curve Analyses.R`文件进行分析，以生成正文及补充材料结果。
2. `Expt 2 MA LT50 input data.csv`：包含第二组实验的数据：将65个MA品系（及其祖先品系）各7只雌性个体分为4（12）个重复培养管，注射DCV后记录存活情况。数据格式为注射后每日存活果蝇数与死亡果蝇数。完整数据说明见'Expt 2 MA LT50 inp...'