Study on the response of soil DNA and RNA viruses and their hosts under warming in Qinling forest.

Here, we investigate the soil viral communities and the virus host interactions after 3 years warming based on soil translocation experiment (from 2200 to 1300 m asl) in Qinling forest soil. Eight soil cores were reinstalled in situ (hereafter referred to as Control) and the other eight were relocated to the low elevation (hereafter referred to as Warm), a warming process by soil translocation. Raw data from metagenome was used to generalize the characteristic of DNA viruses and functions of hosts. Raw data from metatranscriptome was used to generalize the characteristic of RNA viruses. We used the 16S rRNA gene to amplify the V3 V4 region sequences to generalize the abundance of bacterial hosts. The abundance of fungal hosts was detected using high-throughput sequencing of the internal transcribed spacer (ITS1) region. This study suggested that DNA and RNA viruses showed opposite response to soil warming and different changes of viral hosts.

本研究依托秦岭森林土壤的土壤移位实验（将样地从海拔2200米迁移至1300米），探究了3年升温处理后土壤病毒群落与病毒-宿主互作关系。实验共设置16组土壤原状柱：8组原地保留（以下简称对照组），剩余8组被迁移至低海拔区域（以下简称升温组），通过土壤移位实现升温处理。本研究利用宏基因组（metagenome）原始数据解析DNA病毒的特征及其宿主的功能，利用宏转录组（metatranscriptome）原始数据解析RNA病毒的特征；通过扩增16S rRNA基因的V3-V4可变区序列，分析细菌宿主的丰度；通过对内转录间隔区1（ITS1）进行高通量测序，检测真菌宿主的丰度。研究结果表明，DNA病毒与RNA病毒对土壤升温呈现相反的响应模式，且其宿主的动态变化亦存在显著差异。