Mutation of GATA3 in Human Breast Tumors. Homo sapiens

293T epithelial kidney cell line was infected with retrovirus containing pBabe puro empty plasmid or with pBabe puro plasmid containing either GATA3 wild type or mutation R367-L. Stable colonies were selected, grown, and their mRNAs harvested. mRNA from three independent transfections of 293T cells with the empty plasmid was pooled and used as the reference RNA. Dye flips were performed resulting in four arrays. This series also contains two additional arrays (GSM13007 & GSM13008) not in the publication. Keywords: other

本研究以293T上皮肾细胞系为实验材料，分别使用携带pBabe puro空质粒的逆转录病毒，以及携带GATA3野生型或R367-L突变体的pBabe puro质粒的逆转录病毒进行感染。随后筛选获得稳定克隆并扩增培养，收集其信使RNA（mRNA）。将三次独立转染空质粒的293T细胞所提取的mRNA混合，作为参照RNA。通过染料翻转实验得到4张基因芯片数据。本数据集还包含2张未见于原发表文献的芯片（GSM13007与GSM13008）。关键词：其他