Potency of viral pools relative to Ad5 on cognate cell lines.

Potency values less than 1 indicates attenuation relative to Ad5.
The potency of each viral pool that underwent 20 passages of Directed Evolution (Wt20) was compared to the starting pool (Wt0) and to Ad5 on their respective cognate cell line. Viral potencies were measured by MTS assay.

当效价数值小于1时，表明相较于Ad5，该病毒存在减毒特性。针对经20轮定向进化传代得到的各病毒库（Wt20），将其效价分别与起始病毒库（Wt0）以及Ad5在各自对应的同源细胞系中进行对比。病毒效价采用MTS测定法进行检测。