Table1_Analysis of basic pentacysteine6 transcription factor involved in abiotic stress response in Arabidopsis thaliana.XLSX

Background: Abiotic stress is a significant environmental factor that limits plant growth. Plants have complex and diverse mechanisms for dealing with abiotic stress, and different response mechanisms are interconnected. Our research aims to find key transcription factors that can respond to multiple non -biological stress.
Methods: We used gene expression profile data of Arabidopsis in response to abiotic stress, constructed a weighted gene co-expression network, to obtain key modules in the network. The functions and pathways involved in these modules were further explored by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Through the enrichment analysis of transcription factor, the transcription factor that plays an important regulatory role in the key module. Through gene difference expression analysis and building protein interaction networks, the important role of key transcription factors is verified.
Result: In weighted gene co-expression network, identified three gene modules that are primarily associated with cold stress, heat stress, and salt stress. Functional enrichment analysis indicated that the genes in these modules participate in biological processes such as protein binding, stress response, and others. Transcription factor enrichment analysis revealed that the transcription factor Basic Pentacysteine6 (BPC6) plays a crucial regulatory role in these three modules. The expression of the BPC6 gene is dramatically affected under a variety of abiotic stress treatments, according to an analysis of Arabidopsis gene expression data under abiotic stress treatments. Differential expression analysis showed that there were 57 differentially expressed genes in bpc4 bpc6 double mutant Arabidopsis relative to normal Arabidopsis samples, including 14 BPC6 target genes. Protein interaction network analysis indicated that the differentially expressed genes had strong interactions with BPC6 target genes within the key modules.
Conclusion: Our findings reveal that the BPC6 transcription factor plays a key regulatory function in Arabidopsis coping with a variety of abiotic stresses, which opens up new ideas and perspectives for us to understand the mechanism of plants coping with abiotic stresses.

背景：非生物胁迫（Abiotic stress）是限制植物生长的重要环境因素。植物应对非生物胁迫的机制复杂多样，且不同响应机制之间存在相互关联。本研究旨在筛选可响应多种非生物胁迫的关键转录因子（transcription factor）。
方法：本研究使用响应非生物胁迫的拟南芥（Arabidopsis）基因表达谱数据，构建加权基因共表达网络以获取网络中的关键模块。通过基因本体（Gene Ontology, GO）富集分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）富集分析，进一步探究这些模块所涉及的功能与通路。结合转录因子富集分析，筛选在关键模块中发挥重要调控作用的转录因子。最后通过基因差异表达分析与蛋白质相互作用网络构建，验证关键转录因子的重要功能。
结果：在加权基因共表达网络中，共鉴定出三个分别与冷胁迫、热胁迫及盐胁迫显著相关的基因模块。功能富集分析显示，这些模块中的基因参与了蛋白质结合、胁迫应答等生物学过程。转录因子富集分析结果表明，Basic Pentacysteine6（BPC6）在这三个模块中发挥关键调控作用。对非生物胁迫处理下的拟南芥基因表达数据进行分析后发现，BPC6基因的表达在多种非生物胁迫处理下发生显著变化。差异表达分析显示，与野生型拟南芥样本相比，bpc4 bpc6双突变体拟南芥中共存在57个差异表达基因，其中包含14个BPC6靶基因。蛋白质相互作用网络分析表明，在关键模块中，差异表达基因与BPC6靶基因之间存在较强的相互作用。
结论：本研究结果揭示了BPC6转录因子在拟南芥应对多种非生物胁迫过程中发挥关键调控作用，为解析植物应对非生物胁迫的分子机制提供了全新的思路与研究视角。