Effects of phytogalactolipids on drug-resistant colorectal cancer

Drug resistance is one of the major challenges in colorectal cancer (CRC) treatment. Overcoming drug resistance and improving the treatment effect of CRC patients who have developed drug resistance is a crucial issue. Crassocephalum rabens and Bidens pilosa are edible plants that are also commonly used as a folk medicine. This study explored the pharmacological mechanism and therapeutic efficacy of bioactive compounds isolated from Crassocephalum rabens and Bidens pilosa for treating drug-resistant CRC in vitro and in vivo. Overall design: The transcriptional profile of wild-type HT-29 cells was analyzed by RNA-seq following 24h exposure to CRA or BP.

耐药性是结直肠癌（colorectal cancer, CRC）治疗面临的主要挑战之一。克服耐药性、改善耐药结直肠癌患者的治疗效果是一项至关重要的研究课题。野茼蒿（Crassocephalum rabens）与鬼针草（Bidens pilosa）既是可食用植物，同时也常作为民间药用植物使用。本研究探讨了从野茼蒿和鬼针草中分离得到的生物活性成分，用于体外及体内模型治疗耐药结直肠癌的药理机制与治疗功效。实验整体设计：将野生型HT-29细胞经CRA（野茼蒿提取物）或BP（鬼针草提取物）处理24小时后，采用RNA-seq技术分析其转录组表达谱。