High-resolution analysis of parent-of-origin-allelic expression in Arabidopsis seeds. Arabidopsis thaliana

We report the identification of parent-of-origin specific gene expression in Arabidopsis seeds. F1 hybrid seeds were generated by reciprocally crossing Arabidopsis accessions Col-0 and Bur-0. Seeds were harvested at 4 DAP and total RNA was isolated and analyzed by deep sequencing. Overall design: Overall identification of parent-of-origin-allelic expression by analysis of SNP distribution

本研究报道了拟南芥种子中亲本起源特异性基因表达的鉴定成果。研究通过拟南芥生态型Col-0与Bur-0的正反交制备F1杂交种子，于授粉后4天（Days After Pollination, DAP）收获种子，提取总RNA后利用深度测序进行分析。实验设计概述：通过单核苷酸多态性（Single Nucleotide Polymorphism, SNP）分布分析，实现亲本起源等位基因特异性表达的全面鉴定。