Open chromatin regions at the single cell level of CD8+ T cells from the spleens of LCMV-Armstrong or LCMV-Clone 13 infected mice

The profiles of open chromatin regions of CD8+ T cells from LCMV-Armstrong and LCMV-Clone 13 infected mice are known to be distinct from one another. We used Assay for Transposase-Accessible Chromatin using sequencing at the single cell level (scATAC-seq) to analyze these differences in splenic CD8+ T cells of these two infection conditions at Division 1 post-infection. Adoptively transferred CD8+ P14 T cells from the spleen of C57BL/6 mice were isolated by Fluorescence-activated cell sorting (FACS) at day 2 post-infection according to the presence of CD45.1 congenic marker and CFSE signal to isolate cells undergoing their 1st division and analyzed using scATAC-seq.

已知感染淋巴细胞性脉络丛脑膜炎病毒阿姆斯特丹株（LCMV-Armstrong）与克隆13株（LCMV-Clone 13）的小鼠，其CD8阳性T细胞（CD8+ T cells）的开放染色质区域谱存在显著差异。本研究采用单细胞转座酶可及性染色质测序（Assay for Transposase-Accessible Chromatin using sequencing at the single cell level，scATAC-seq），对两种感染模型下小鼠感染后第1细胞分裂周期的脾脏CD8阳性T细胞的染色质开放差异展开分析。研究人员于感染后第2天，通过荧光激活细胞分选（Fluorescence-activated cell sorting，FACS）分离自C57BL/6小鼠脾脏获取的过继转移CD8阳性P14 T细胞，依据CD45.1同源标记与CFSE信号筛选处于首次细胞分裂的细胞，并采用scATAC-seq完成后续分析。