mbctools: A User-Friendly Metabarcoding and Cross-Platform Pipeline for Analyzing Multiple Amplicon Sequencing Data across a Large Diversity of Organisms

Test data for the mbctools wrapper. Context and description: Chagas disease (CD) is a zoonotic parasitic disease caused by Trypanosoma cruzi, mainly transmitted to mammals through feces and urine of triatomines known as kissing bugs (Hemiptera: Reduviidae). Triatoma dimidiata is the main vector of CD in the Yucatan Peninsula (YP). To better understand ecological interactions shaping transmission cycles of T. cruzi in a sylvatic ecotope of the YP, a metabarcoding approach was used to simultaneously identify vector blood meals, gut microbiome composition, genetic diversity, as well as T. cruzi genetic diversity from a sample of 33 T. dimidiata individuals collected in 2019 by the owner of an isolated rural dwelling on a conserved sylvatic site of the Balam Kú State Reserve (Campeche, Mexico). Infection prevalence with T. cruzi was 27.3% (9/33). We identified thirteen blood-meal sources (the most prevalent being Homo sapiens, Sus scrofa, Mus musculus), six bacterial Orders (Bacillales, Oceanospirillales and Micrococcales being the most abundant), two T. cruzi DTUs (TcI and TcIV), as well as two T. dimidiata genetic subgroups (ITS-2 Haplogroups 2 and 3).

mbctools包装器测试数据集。背景与说明：恰加斯病（Chagas disease, CD）是由克氏锥虫（Trypanosoma cruzi）引发的人畜共患寄生虫病，主要通过被称为接吻虫的猎蝽（半翅目：猎蝽科）的粪便与尿液传播至哺乳动物。二带喙猎蝽（Triatoma dimidiata）是尤卡坦半岛（Yucatan Peninsula, YP）恰加斯病的主要传播媒介。为更深入解析尤卡坦半岛一处野生森林生境中调控克氏锥虫传播循环的生态互作机制，本研究采用宏条形码（metabarcoding）技术，对2019年在墨西哥坎佩切州巴拉姆库州立保护区一处受保护的原始森林区域内，由某偏远乡村住宅所有者采集的33只二带喙猎蝽样本，同步完成了媒介宿主血液溯源、肠道微生物组组成、种群遗传多样性以及克氏锥虫遗传多样性的鉴定。样本的克氏锥虫感染率为27.3%（9/33）。本研究共鉴定出13种血液餐来源（占比最高的为智人（Homo sapiens）、野猪（Sus scrofa）、小家鼠（Mus musculus））、6个细菌目（丰度最高的类群为芽孢杆菌目（Bacillales）、海洋螺菌目（Oceanospirillales）与微球菌目（Micrococcales））、2种克氏锥虫离散型分类单元（Discrete Typing Units, DTUs）（TcI与TcIV），以及2个二带喙猎蝽遗传亚群（ITS-2单倍群2与3）。