miRNA expression in rat medial entorhinal cortex layers during postnatal development

To examine how miRNAs differ between medial entorhinal cortex layers during postnatal development, we analyzed the miRNA expression in layer II and the deep layers (III-VI) at postnatal ages P2, P9, P23 and P45 in wildtype Long Evans rats. The expression of the two most significantly differentially expressed miRNAs were validated by in situ hybridization.为探究出生后发育阶段内侧内嗅皮层各皮层层间微小RNA（miRNAs）的表达差异，我们对野生型Long Evans大鼠在出生后第2天（P2）、第9天（P9）、第23天（P23）及第45天（P45）时的第二层皮层与深层皮层（III-VI层）的微小RNA表达情况进行了分析。随后通过原位杂交（in situ hybridization）对两种差异表达最为显著的微小RNA的表达水平进行了验证。