Optimization of genetically encoded GABA indicator

The GENIE Project Team at HHMI Janelia has developed a second-generation genetically encoded GABA indicator (Fig. 1a), in collaboration with Jonathan Marvin and Loren Looger. The sensor is based on iGABASnFR (Marvin, J.S., Shimoda, Y., Magloire, V. et al. A genetically encoded fluorescent sensor for in vivo imaging of GABA. Nat Methods 16, 763–770 (2019). https://doi.org/10.1038/s41592-019-0471-2), and has the following features, tested extensively in cultured neurons.霍华德·休斯医学研究所（HHMI）詹妮莉亚研究园区的GENIE项目团队，与乔纳森·马文（Jonathan Marvin）、洛伦·卢格（Loren Looger）合作，开发了第二代遗传编码型γ-氨基丁酸（GABA）指示剂（图1a）。该传感器基于iGABASnFR（Marvin, J.S., Shimoda, Y., Magloire, V. 等. 用于活体γ-氨基丁酸成像的遗传编码荧光传感器. 《自然·方法学》, 16, 763–770 (2019). https://doi.org/10.1038/s41592-019-0471-2），具备以下特性，且相关特性已在培养神经元中完成了广泛的性能测试。