Preliminary evaluation of a qPCR for Toxplasma DNA in meat, an ELISA and agglutination test for anti-Toxoplasma antibodies in serum, and filter strips for field blood collection, using samples collected from northern wildlife between 2006 and 2010

The data set includes sample identification, species, collection location (described using one or more of the following: name of a prominent geographic feature, nearest community, latitude and longitude), date of collection, date of testing, age, sex, sample type, results. Protocols are described and consist of a qPCR to detect Toxoplasma DNA in meat, a multispecies ELISA using a protein A/G conjugate and a commercial agglutination test kit (MAT) - both to detect anti-Toxoplasma antibodies in serum, and a filter strip procedure (Nobuto) for blood collection under field conditions. Limitations of preliminary results are discussed including the major factor in preventing timely evaluation of new diagnostic techniques for toxoplasmosis in wildlife which is the lack of known infected and negative animals from which to obtain control samples.

本数据集涵盖样本标识、物种信息、采集地点（可通过以下任意一种或多种方式标注：标志性地理地貌名称、最近居民区、经纬度）、采集日期、检测日期、样本年龄、性别、样本类型及检测结果。数据集附带完整实验方案，包括用于检测肉类中弓形虫（Toxoplasma）DNA的实时荧光定量PCR（qPCR）技术、采用蛋白A/G结合物的多物种酶联免疫吸附试验（ELISA），以及可同时检测血清中抗弓形虫抗体的商用凝集试验试剂盒（MAT），另包含野外环境下采血所用的滤纸条采样流程（Nobuto）。研究同时讨论了初步结果的局限性，其中制约野生动物弓形虫病新型诊断技术及时开展评估的核心瓶颈，是缺乏已知感染状态（阳性与阴性）的对照动物样本来源。