Targeting Immune-Fibroblast Crosstalk in Myocardial Infarction and Cardiac Fibrosis II

To examine whether commonly used human cultured fibroblast systems model human cardiac fibroblasts, we tested three different fibroblast cell preparations: primary human cardiac fibroblasts (NHCF), human dermal fibroblasts (NHDF), and immortalized human cardiac fibroblasts (iHCF). Overall design: Each fibroblast cell line was treated with vehicle, TGF-ß, or IL-1ß for 72 hours and scRNAseq performed. Multiple biological and technical replicates were included, individually hash tagged, and pooled to minimize any potential batch effects.

为探究常用的人类培养成纤维细胞系统能否模拟人类心脏成纤维细胞，我们测试了三种不同的成纤维细胞制剂：原代人类心脏成纤维细胞（primary human cardiac fibroblasts, NHCF）、人类皮肤成纤维细胞（human dermal fibroblasts, NHDF）以及永生化人类心脏成纤维细胞（immortalized human cardiac fibroblasts, iHCF）。整体实验设计如下：将每种成纤维细胞系分别以溶剂对照、转化生长因子β（TGF-β）或白细胞介素1β（IL-1β）处理72小时，随后进行单细胞RNA测序（single-cell RNA sequencing, scRNAseq）。本实验包含多个生物学重复与技术重复，所有样本均经单独哈希标签标记后混合，以尽可能降低潜在的批次效应。