Gene Expression Profiling of SPOP Knocked Down Cell

SPOP is one of the most frequent mutated genes in prostate cancer. In the current study, we identified CCNE1 as its substrate. SPOP directly interacts with CCNE1, poly-ubiquitinates CCNE1 in vivo and in vitro, and represses cancer-related phenotypes induced by CCNE1 expression. Thus, we reveal a new mechanism for SPOP in repressing prostate cancer tumorigenesis. SPOP was knocked down by siRNA in DU145 and 769-P, the gene expression profile was studied by RNA sequencing

SPOP是前列腺癌中最常见的突变基因之一。本研究中，我们鉴定出CCNE1为其靶底物。SPOP可直接与CCNE1结合，在体内外对CCNE1进行多泛素化修饰，并抑制CCNE1表达所诱导的肿瘤相关表型。由此，我们揭示了SPOP抑制前列腺癌发生的全新机制。我们通过siRNA在DU145与769-P细胞中敲低SPOP，并利用RNA测序技术分析其基因表达谱。