Global temporal gene expression of Nematostella vectensis in-situ. Global temporal gene expression of Nematostella vectensis in-situ

We assessed genome-wide temporal transcript expression patterns in the sea anemone, Nematostella vectensis, in Great Sippewissett Marsh in Massachusetts, where anemones experienced a natural light cycle with intensity varying from 0-200 lum/ft2, daily temperature fluctuations of ~9C. We measured ‘in situ’ gene expression from recaptured anemones every hour from 0800 to 1700 and identified six time-dependent gene clusters, represented by several genes involved in metabolism, stress, and transcription-translation related functions. Overall design: We measured gene expression from anemones every hour from 0800 to 1700 for a total of 10 time points in both single individuals and pooled samples

本研究以美国马萨诸塞州锡佩威西特大沼泽中的星状海葵（Nematostella vectensis）为研究对象，分析其全基因组范围的时间动态转录表达模式。该生境下海葵所处的自然光照周期强度区间为0~200 lum/ft2，每日温度波动约9℃。研究人员于每日8:00至17:00每小时对重捕获的海葵开展原位（in situ）基因表达检测，最终鉴定得到6个时间依赖性基因簇，此类基因簇由参与代谢、胁迫及转录翻译相关功能的多个基因所代表。
实验设计概述：本研究于每日8:00至17:00每小时采集海葵样本，共计设置10个时间点，分别采用单个体样本与混合样本进行基因表达检测。