Human breast cancer SKBr-3 cells: trastuzumab-resistant vs. parental

Human breast cancer SKBr-3 cells selected with trastuzumab for 6 month compared with parental SKBr-3 cells; Goal was to screen for microRNAs involved in development of trastuzumab resistance. Human breast cancer SKBr-3 cells were continuously cultured in the presence of  5µg/ml trastuzumab for 6 months, and the resulting alive cells were regarded as trastuzumab-resistant. Total RNAs were prepared from these cells and cells cultured in parallel in control media, and were subjected to hybridization on the miRCURY LNA Array (Exiqon, version 11.0).

本数据集对比了经曲妥珠单抗（trastuzumab）持续处理6个月筛选获得的人乳腺癌SKBr-3细胞与亲本SKBr-3细胞，研究目标为筛选参与曲妥珠单抗耐药发生过程的微小核糖核酸（microRNAs）。具体实验流程如下：将人乳腺癌SKBr-3细胞置于含5μg/ml曲妥珠单抗的培养基中连续培养6个月，存活的细胞即被定义为曲妥珠单抗耐药细胞；分别从该耐药细胞及同期于对照培养基中培养的亲本细胞中提取总RNA，随后采用miRCURY LNA Array（Exiqon，11.0版本）进行杂交检测。