Enhancer profiling in Esrrb-/- ES cells [ChIP-seq]. Enhancer profiling in Esrrb-/- ES cells [ChIP-seq]

We uncover a pivotal role for ESRRB in demarcating ESC-specific enhancer units, and propose that ESRRB’s developmentally-regulated extinction precipitates their decommissioning with impact on the rewiring of the pluripotency transcriptional programme upon embryo implantation Overall design: Profiling H3K27ac and Med1 occupancy through ChIP-seq

我们揭示了雌激素相关受体β（ESRRB）在界定胚胎干细胞（ESC，Embryonic Stem Cell）特异性增强子单元中的关键作用，并提出：ESRRB受发育调控的表达沉默会促使这些增强子单元失活，进而影响胚胎着床时多能性转录程序的重布线。实验设计概述：通过染色质免疫共沉淀测序（ChIP-seq，Chromatin Immunoprecipitation Sequencing）检测组蛋白H3赖氨酸27乙酰化（H3K27ac）与中介体复合物亚基1（Med1）的染色质结合占据情况。