The interferon/STAT1 signaling axis is a common feature of tumor-initiating cells in breast cancer

Gene expression profile of STAT-signaling tumor-initiating cells (TIC) in breast xenograft models Overall design: Cell line- and patient-derived xenografts transduced with a STAT reporter or STAT lineage tracing system were FACS-enriched according to the presence/absence of EGFP (STAT reporter) and/or the presence of mNeptune2 or dsRed (lineage tracing system)

乳腺癌异种移植模型中信号转导与转录激活因子（STAT）信号通路相关肿瘤起始细胞（tumor-initiating cells, TIC）的基因表达谱
实验设计概况：对转导STAT报告基因或STAT谱系示踪系统的细胞系来源异种移植瘤及患者来源异种移植瘤，依据增强型绿色荧光蛋白（Enhanced Green Fluorescent Protein, EGFP，STAT报告基因）的有无、以及mNeptune2或dsRed（谱系示踪系统标记物）的表达情况，通过荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）完成细胞富集