Early life microbiome perturbation in male mice by cohousing with mice from another vendor

Weanling mouse pups from The Jackson Laboratories or from Taconic Farms were placed in sex-specific cages either with other mice from the same vendor (same-housed) or with mice from the opposite vendor (cohoused). Mice were maintained with these cagemates until they were 10-12 weeks of age. Fecal pellets were then harvested 16S sequencing of fecal DNA was performed.

来自杰克逊实验室（The Jackson Laboratories）或塔科尼奇农场（Taconic Farms）的断乳仔鼠被安置于按性别划分的笼具中，分别与来自同一供应商的其他小鼠（same-housed）或来自另一供应商的小鼠（cohoused）共同饲养。所有小鼠均与同笼伙伴一同饲养至10至12周龄。随后收集粪便颗粒，并对粪便DNA开展16S测序。