Glycine max cultivar:NJCMS5A Raw sequence reads. Glycine max cultivar:NJCMS5A

Comparative genome-wide DNA methylation profiles of the soybean CMS line NJCMS5A and its maintainer NJCMS5B were completed by whole-genome bisulfite sequencing. 3,527 differentially methylated regions (DMRs) and 485 differentially methylated genes (DMGs), including 353 high-credible methylated genes, 56 methylated genes coding unknown protein and 76 novel methylated genes with no known function were identified. Finally, 14 key DMGs were identified as potentially related to CMS in soybean.

本研究采用全基因组亚硫酸氢盐测序（whole-genome bisulfite sequencing）技术，完成了大豆细胞质雄性不育（cytoplasmic male sterility, CMS）系NJCMS5A及其保持系NJCMS5B的全基因组DNA甲基化比较图谱分析。研究共鉴定出3527个差异甲基化区域（differentially methylated regions, DMRs）及485个差异甲基化基因（differentially methylated genes, DMGs），其中涵盖353个高可信度甲基化基因、56个编码未知蛋白的甲基化基因，以及76个功能未明确的新型甲基化基因。最终，筛选得到14个关键差异甲基化基因，其潜在关联大豆细胞质雄性不育性状。