Gene expression in human breast cancer T47D cells associated with expression of PTH-AS, a lncRNA downstream of parathyroid hormone. Gene expression in human breast cancer T47D cells associated with expression of PTH-AS, a lncRNA downstream of parathyroid hormone

To comprehensively analyze the effect of PTH-AS on gene expression, microarray analysis was performed using human breast cancer T47D cells expressing PTH-AS. The gene expression levels of full-length PTH-AS expressing T47D cells (T47D-PA6) and vector control (T47D-VC1) or incomplete PTH-AS expressing T47D cells (T47D-PA5) were compared. As a result, 1453 genes were differentially expressed in his T47D-PA6 compared to T47D-VC1. Except for genes whose expression was altered in T47D-PA5 cells expressing incomplete PTH-AS, PTH-AS expression in T47D cells upregulated 914 genes and downregulated 600 genes. Many of the altered genes have been classified as immune-related biological processes. Particularly striking was the significant upregulation of JAK-STAT pathway-related genes, including the interferon-related DNA damage resistance signature (IRDS) that promotes cancer. Overall design: To eliminate artifacts from extracellular gene transfer, full-length PTH-AS-expressing T47D cells (T47D-PA6) were compared with vector control (T47D-VC1) as well as T47D cells expressing incomplete PTH-AS (T47D-PA5) .

为全面解析PTH-AS对基因表达的调控作用，本研究针对表达PTH-AS的人乳腺癌T47D细胞开展了微阵列分析。本研究比较了三类细胞的基因表达水平：表达全长PTH-AS的T47D细胞（T47D-PA6）、空载体对照细胞（T47D-VC1），以及表达截短型PTH-AS的T47D细胞（T47D-PA5）。结果显示，相较于T47D-VC1，T47D-PA6细胞中共鉴定出1453个差异表达基因；在排除T47D-PA5中因截短型PTH-AS表达引发的表达改变基因后，T47D细胞中PTH-AS的表达可上调914个基因、下调600个基因。大量差异表达基因被归类为免疫相关生物学过程，其中尤为突出的是JAK-STAT信号通路相关基因的显著上调，包括可促进肿瘤发生的干扰素相关DNA损伤抵抗特征（interferon-related DNA damage resistance signature, IRDS）。实验总体设计：为排除外源基因转移带来的实验系统误差，本研究将表达全长PTH-AS的T47D细胞（T47D-PA6）分别与空载体对照细胞（T47D-VC1）、表达截短型PTH-AS的T47D细胞（T47D-PA5）进行了对照比较。