Screening and molecular mechanism research on bile microRNAs associated with chemotherapy efficacy in perihilar cholangiocarcinoma

This dataset includes high-throughput sequencing data of bile microRNAs (miRNAs) and mRNAs from patients with advanced perihilar cholangiocarcinoma (pCCA) treated with hepatic arterial infusion chemotherapy (HAIC) using oxaliplatin (OXA) and 5-fluorouracil (5-Fu). The study aimed to identify miRNAs associated with chemotherapy resistance in pCCA patients. Bile samples were collected from patients with varying survival outcomes, and sequencing data were used to identify differentially expressed miRNAs and mRNAs. Key miRNAs, such as miR-532-3p, miR-1250-5p, and miR-4772-5p, were found to correlate with patient survival, and miR-532-3p was shown to promote chemotherapy resistance through negative regulation of SPARC. This dataset may help in further understanding the molecular mechanisms of chemotherapy resistance and identifying potential biomarkers or therapeutic targets for pCCA.

本数据集包含接受以奥沙利铂（oxaliplatin，OXA）联合5-氟尿嘧啶（5-fluorouracil，5-Fu）方案行肝动脉灌注化疗（hepatic arterial infusion chemotherapy，HAIC）的晚期肝门部胆管癌（perihilar cholangiocarcinoma，pCCA）患者的胆汁微小RNA（microRNAs，简称miRNAs）与信使RNA（messenger RNA，简称mRNA）高通量测序数据。本研究旨在筛选与pCCA患者化疗耐药相关的miRNAs。研究收集了不同生存结局患者的胆汁样本，通过测序数据鉴定差异表达的miRNAs与mRNA。研究发现miR-532-3p、miR-1250-5p及miR-4772-5p等关键miRNAs与患者生存情况显著相关，且miR-532-3p可通过负调控SPARC促进化疗耐药。本数据集可为进一步阐明化疗耐药的分子机制提供数据支撑，同时有助于筛选pCCA的潜在生物标志物或治疗靶点。