Proteolytic activity of C-SVMP on C1-Inh and analysis of its regulatory activity.

[A] Human purified C1-Inh samples (3 µg) were incubated with purified C-SVMP (0.5 µg) or Bothrops venom (1 µg) in the presence or absence of 1,10 phenanthroline (Phe - 15 mM). [B] Alternatively, samples of purified human C1-Inh (1 µg) were incubated with increasing concentrations of C-SVMP (0.5 µg, 1 µg and 2 µg). The cleavage was visualized by SDS-PAGE (10%) under reducing conditions followed by silver staining. [C] C1-Inh activity was determined using the MicroVue ELISA kit (***p<0.001). Data are representative of two separate experiments.

[A] 经人源纯化的C1抑制物（C1-Inh）样本（3 µg）分别与纯化的C-SVMP（0.5 µg）或矛头蛇毒液（Bothrops venom，1 µg）在含有或不含1,10-邻菲啰啉（Phe，15 mM）的体系中进行孵育。
[B] 此外，将1 µg经人源纯化的C1-Inh样本与梯度浓度的C-SVMP（0.5 µg、1 µg及2 µg）孵育。蛋白裂解产物通过还原条件下的10%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离，随后采用银染法显色。
[C] 采用MicroVue酶联免疫吸附实验（ELISA）试剂盒检测C1-Inh活性（***p<0.001）。实验数据均为两次独立重复实验的代表性结果。