Mass spectrometry-based profiling of HDF, HDF Vim -/-, and their EVs, and the impact of these EVs on MCF7 and MCF10A proteomes

Vimentin is a well-established intracellular regulator of epithelial-mesenchymal transition (EMT). Recent studies have highlighted the role of extracellular vimentin from fibroblast-derived extracellular vesicles (EVs) in promoting wound healing and other functions. Building on these findings, we investigated the protein cargo of wild-type (WT) and vimentin-knockout (Vim -/-) human dermal fibroblasts (HDFs) and their EVs, aiming to determine whether extracellular vimentin influences EMT. Additionally, we assessed the impact of EVs from WT and Vim -/- HDFs on the proteomes of MCF7 and MCF10A cells

波形蛋白（Vimentin）是公认的细胞内上皮间质转化（epithelial-mesenchymal transition, EMT）调控因子。近期研究凸显了成纤维细胞来源细胞外囊泡（extracellular vesicles, EVs）所携带的细胞外波形蛋白在促进伤口愈合及其他生理功能中的作用。基于上述发现，本研究对野生型（wild-type, WT）及波形蛋白敲除（Vimentin-knockout, Vim -/-）人真皮成纤维细胞（human dermal fibroblasts, HDFs）及其分泌的细胞外囊泡的蛋白质载荷进行了分析，旨在明确细胞外波形蛋白是否会对上皮间质转化产生影响。此外，本研究还评估了野生型与Vim -/-人真皮成纤维细胞来源的细胞外囊泡对MCF7及MCF10A细胞蛋白质组的影响。