Unveiling the Clinical Significance of RNA Pseudouridylation in Colorectal Cancer

Colorectal cancer is a common malignant tumor worldwide, and its morbidity and mortality are increasing year by year. RNA pseudouracil modified synthetase is closely related to the development of colorectal cancer, but the whole transcriptomic level of pseudouridine(?) in colorectal cancer has not been fully explored. This study examined the extent of RNA ? modification in 21 pairs of colorectal cancer tissues and adjacent normal tissues, as well as 10 blood samples from patients, utilizing RNA ? modification sequencing technology. These findings underscore the significance of RNA ? modification events in colorectal cancer and offer insights into its application for clinical detection and treatment. Overall design: This study comprehensively assessed the alterations and consistency of RNA ?modification in colorectal cancer tissues and blood samples from 21 patients recruited at Zhongnan Hospital of Wuhan University, along with ? sequencing data from 10 colorectal cancer patients and 13 healthy controls. Utilizing the ? sequencing results, we investigated differential pseudouridylated genes across various RNA types and elucidated their correlation with clinical characteristics

结直肠癌（Colorectal cancer）是全球范围内常见的恶性肿瘤，其发病率与死亡率逐年攀升。RNA假尿嘧啶修饰合酶（RNA pseudouracil modified synthetase）与结直肠癌的发生发展密切相关，但目前结直肠癌中假尿苷（pseudouridine）的全转录组修饰水平尚未得到充分探索。本研究采用RNA假尿苷修饰测序技术，对21对结直肠癌组织及配对癌旁正常组织、10例患者的血液样本中的RNA假尿苷修饰程度进行了检测。本研究结果凸显了RNA假尿苷修饰事件在结直肠癌中的重要意义，为其在临床检测与治疗中的应用提供了新的研究思路。
总体实验设计：本研究全面评估了武汉大学中南医院招募的21例结直肠癌患者的癌组织与血液样本中RNA假尿苷修饰的变化情况与一致性，并纳入了10例结直肠癌患者与13例健康对照的假尿苷测序数据。基于上述测序结果，本研究分析了不同RNA类型间的差异假尿苷化基因，并阐明了其与临床特征的相关性。