Pilot-Scale Depuration Trial Data

We conducted several depuration trials to reduce <i>Vibrio parahaemolyticus </i>in three species of oyster: <i>Crassostrea gigas</i>, <i>C. sikamea</i>, and <i>C. virginica</i>. We also compared depuration of non-pathogenic and pathogenic isolates of <i>V. parahaemolyticus.</i> Depuration trials were conducted at 11 or 13 °C for 5-7 days, with oysters sampled daily to evaluate <i>V. parahaemolyticus</i> concentrations. Oysters were homogenized and the homogenate cultured to evaluate <i>V. parahaemolyticus </i>concentrations. Reductions in <i>V. parahaemolyticus</i> were determined by plating homogenized oyster tissue on TCBS agar, a selective media for <i>Vibrio</i>.Depuration significantly reduced the concentrations of <i>V. parahaemolyticus</i> in all three species of oyster tested, and non-pathogenic and pathogenic <i>V. parahaemolyticus</i> isolates were reduced at an equivalent rate, allowing for their use in future depuration validation studies.

本研究开展多组贝类净化试验，以降低三种牡蛎体内的副溶血性弧菌（Vibrio parahaemolyticus），受试牡蛎分别为长牡蛎（Crassostrea gigas）、西卡莫牡蛎（C. sikamea）以及美洲牡蛎（C. virginica）。本研究同时比较了副溶血性弧菌非致病分离株与致病分离株的净化效果。贝类净化试验于11℃或13℃条件下开展，持续5至7天，每日采集牡蛎样本以检测副溶血性弧菌含量。将牡蛎组织均质化后，对均质物进行培养以测定副溶血性弧菌含量。通过将均质化牡蛎组织涂布于硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂（TCBS agar，弧菌选择性培养基）上，可确定副溶血性弧菌的消减情况。净化处理可显著降低受试三种牡蛎体内的副溶血性弧菌含量，且副溶血性弧菌非致病株与致病株的消减速率相当，该两类分离株可用于后续的净化验证研究。