SH1044430.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 为基于DNA的物种假说（Species Hypotheses，SH）的界定、识别、传播与相关研究提供了统一的标准化路径。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一组动态距离阈值（<0.5%~3.0%）被聚类至近似物种水平。所有物种假说均会被赋予唯一且稳定的数字对象唯一标识符（DOI，Digital Object Identifier）格式的名称，其分类学与生态学注释信息将通过分布式网络第三方注释项目完成核验。每个物种假说都会结合其包含的所有序列的鉴定结果，尽可能匹配至对应的分类单元名称及其所属分类层级（如门、纲、目等）。研究人员会选取一条经自动或手动指定的序列作为该物种假说的代表序列。上述代表序列已发布于https://unite.ut.ee/repository.php，可供科研社区用于本地序列相似性搜索、新一代测序分析流程等科研场景。随着公共真菌ITS序列的数量持续增长，该系统与配套数据集将自动完成更新。