Spatiotemporal transcriptional atlas of lung adenocarcinoma from adenocarcinoma in situ to invasive carcinoma [scRNA-seq]. Spatiotemporal transcriptional atlas of lung adenocarcinoma from adenocarcinoma in situ to invasive carcinoma [scRNA-seq]

To illuminate the evolutionary trajectory of LUAD from AIS to IAC, high-throughput scRNA-seq and ST data were generated and integrated to create a large-scale, single-cell spatiotemporal atlas of LUAD. We compiled a multi-omics atlas of the early-stage LUAD invasion process that reflects the heterogeneity of cancer cells, the competitive polyclonal origin of LUAD, signalling interactions between cancer cells and the TME, and the pseudo-chronological nature of LUAD invasion. The spatial distribution characteristics of LUAD cells revealed the spatial heterogeneity of LUAD and the mechanism of spatial immune escape in LUAD, which provides strong evidence supporting clinical diagnosis and surgical intervention at the single-cell level. Overall design: Nine resected samples (TD1-9, three each from AIS, MIA and IAC cases) from nine treatment-naïve patients were collected for droplet-based scRNA-seq. In parallel, six paired samples were subjected to ST

为阐明肺腺癌（LUAD）从原位腺癌（AIS）进展至浸润性肺腺癌（IAC）的演化轨迹，本研究生成并整合了高通量单细胞RNA测序（scRNA-seq）与空间转录组（ST）数据，构建了大规模肺腺癌单细胞时空图谱。本研究构建了早期肺腺癌侵袭进程的多组学图谱，该图谱可反映癌细胞异质性、肺腺癌的多克隆竞争起源、癌细胞与肿瘤微环境（TME）间的信号互作，以及肺腺癌侵袭的拟时序特征。肺腺癌细胞的空间分布特征揭示了肺腺癌的空间异质性与空间免疫逃逸机制，为单细胞层面的临床诊断与手术干预提供了有力依据。整体实验设计：本研究纳入9例初治患者的9份切除样本（编号TD1-9，分别包含3例原位腺癌、3例微浸润性腺癌（MIA）与3例浸润性肺腺癌病例样本），采用液滴法开展单细胞RNA测序。与此同时，对6份配对样本进行空间转录组（ST）检测。