Polycomb repressive complex 1 (PRC1) role in skin epithelium development [HF I53A]

Polycomb repressive complexes (PRC) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that despite its well-established repressor functions, PRC1 binds to both silent and active genes. Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification.多梳抑制复合体1和2（Polycomb repressive complexes, PRC1、PRC2）是调控细胞身份的关键染色质调控因子。作为多梳介导调控的核心执行因子，PRC1以多种亚复合体形式存在，兼具依赖催化活性的H2AK119单泛素化（H2AK119ub）功能与不依赖催化活性的调控活性。尽管PRC1的转录抑制功能已得到广泛证实，本研究发现其可同时结合沉默基因与活跃基因。通过体内功能丧失实验，我们证实全局PRC1功能对皮肤发育及干细胞（stem cell, SC）特化具有不可或缺的作用。