The major diversification of Vγ1.1+ and Vγ2+ thymocytes in mice occurs after commitment to the γδ T-cell lineage

Adult murine gammadelta thymocytes can be divided into two major subsets based on their expression of TCRVgamma1.1 or TCRVgamma2, respectively. gammadelta thymocytes mature through distinct stages characterized by CD24 and CD73 in the following order: immature uncommitted (CD24+CD73-), immature committed (CD24+CD73+) and mature (CD24-CD73+). In this dataset we have sorted gammadelta thymocytes into 6 distinct populations based on subset and developmental stage and analyzed their gene expression. These data were used to identify 3400 differentially expressed genes during gammadelta thymocyte development and to show that the two major adult gammadelta T cells follow a similar path through early development and mainly diversify after commitment to the gammadelta T cell lineage. 22 samples (6 populations 3-4 biological replicates) were analyzed for differential expression to identify genes shared between cells of the different subsets at each developmental stage.

成年小鼠γδ胸腺细胞（gammadelta thymocytes）可根据其分别表达的TCRVγ1.1（TCRVgamma1.1）与TCRVγ2（TCRVgamma2）分为两大亚群。γδ胸腺细胞的成熟过程以CD24与CD73为特征划分为不同发育阶段，依次为：未成熟未定向（CD24+CD73⁻）、未成熟已定向（CD24+CD73⁺）以及成熟（CD24⁻CD73⁺）。本数据集根据细胞亚群与发育阶段将γδ胸腺细胞分为6个独立细胞群，并对其基因表达进行了分析。本研究依托该数据集的基因表达数据，鉴定出了γδ胸腺细胞发育过程中的3400个差异表达基因，并证实两大成年γδ T细胞在早期发育阶段遵循相似的发育路径，且主要在定向为γδ T细胞谱系后发生分化多样化。本研究共分析22份样本（6个细胞群各设置3-4次生物学重复），通过差异表达分析鉴定出不同发育阶段下各亚群细胞共有的基因。